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Choose collection locationVitamins & Minerals · Individual laboratory test
Total 25-hydroxyvitamin D is the usual blood test for vitamin D status. It can help investigate bone or mineral concerns or follow treatment when indicated.
Clinical authorDr Nirupama SabhapathySpecialist Clinical Pathology
Understanding the test
Also called 25-Hydroxyvitamin D
A clinician may request this measurement for a bone or mineral problem, suspected malabsorption or follow-up of an agreed vitamin D treatment plan.
25-hydroxyvitamin D is the circulating form generally used to evaluate vitamin D stores.
The clinician can compare the result with prior measurements, supplement use and the reason for testing.
Before your sample
No fasting is generally needed. Report the strength and frequency of vitamin D supplements and the most recent dose, especially when checking treatment response.
A clinician takes a small sample of blood from a vein. The laboratory uses serum, the liquid separated from the blood after it clots.
Smart Report
24 hoursLaboratory turnaround
The result should be interpreted using the units on the report. The clinician may consider calcium, kidney health and current supplements before recommending changes.
From sample to follow-up

Choose your location, add the test to your care bag and select a time. If you are booking for someone else, assign the test to them in checkout.
Choose collection locationLook for Vitamin D, 25 Hydroxy (25-OH), Total in your report. Use the sample type and collection time when comparing it with an earlier report.
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Keep the result, units and vitamin D supplement history together, then ask whether the level changes the agreed follow-up plan.
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Patient questions
No. The active form is a different measurement with different indications. For most questions about vitamin D status, clinicians request 25-hydroxyvitamin D.
Living in a sunny place does not tell you how much vitamin D your body makes. Time spent indoors, covered skin and deeper skin pigmentation can reduce production. Diet and poor absorption can matter too. Keep protecting your skin from sunburn while you discuss the result.
Often none, which is why it is usually found on a blood test. Marked or long-standing deficiency can cause bone pain, aching or weak muscles and, in children, poor bone growth. Tiredness is common in people with low vitamin D, but a low result alone does not prove it is the cause.
Yes, if both tests measured total 25-hydroxyvitamin D. Multiply ng/mL by 2.5 to get nmol/L. For example, 20 ng/mL and 50 nmol/L are the same concentration. Check the units before treating a change in the number as a change in your vitamin D.
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Written by Dr Nirupama Sabhapathy, Specialist Clinical Pathology. Medically reviewed by Dr Joyce Jose, Specialist Clinical Pathology, on .
Catalogue information and AED price checked on 2 November 2026. Clinical suitability, preparation, collection arrangements and turnaround are confirmed for your selected test.