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Vitamins & Minerals · Individual laboratory test

Vitamin D, 25 Hydroxy (25-OH), Total

Total 25-hydroxyvitamin D is the usual blood test for vitamin D status. It can help investigate bone or mineral concerns or follow treatment when indicated.

Price for this test on its ownAED 150Test price AED 50
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Dr Nirupama SabhapathyClinical authorDr Nirupama SabhapathySpecialist Clinical Pathology
Sunlight illuminates the porous interior of a bone cross section.
Sample
Serum
Laboratory turnaround
24 hours
Collection
Dubai
Laboratory
Intel Lab
Vitamin D, 25 Hydroxy (25-OH), Total specimen guide showing serum.

Understanding the test

What does the Vitamin D (25-OH) test measure?

Also called 25-Hydroxyvitamin D

A clinician may request this measurement for a bone or mineral problem, suspected malabsorption or follow-up of an agreed vitamin D treatment plan.

When it may be requested

Assessing vitamin D status

25-hydroxyvitamin D is the circulating form generally used to evaluate vitamin D stores.

Monitoring an existing plan

The clinician can compare the result with prior measurements, supplement use and the reason for testing.

Before your sample

Prepare for collection

SpecimenSerum
Your checklist0 of 2 ready

No fasting is generally needed. Report the strength and frequency of vitamin D supplements and the most recent dose, especially when checking treatment response.

A clinician takes a small sample of blood from a vein. The laboratory uses serum, the liquid separated from the blood after it clots.

Smart Report

Understanding your result

24 hoursLaboratory turnaround

Intel LabResult guide

Vitamin D, 25 Hydroxy (25-OH), Total

Reading your result

The result should be interpreted using the units on the report. The clinician may consider calcium, kidney health and current supplements before recommending changes.

What can affect it

  • A level alone does not determine the right supplement dose for everyone.

From sample to follow-up

Your testing journey

Intel Lab specimen collection kit

Arrange your collection

Choose your location, add the test to your care bag and select a time. If you are booking for someone else, assign the test to them in checkout.

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Intel Lab
Laboratory testVitamin D, 25 Hydroxy (25-OH), Total
Specimen
Serum
Turnaround
24 hours

Open your laboratory report

Look for Vitamin D, 25 Hydroxy (25-OH), Total in your report. Use the sample type and collection time when comparing it with an earlier report.

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clinician and adult patient discussing laboratory results

Discuss the next step

Keep the result, units and vitamin D supplement history together, then ask whether the level changes the agreed follow-up plan.

Clinical expertise

Your laboratory team

Patient questions

Before you book

Is this the same as the active form of vitamin D?

No. The active form is a different measurement with different indications. For most questions about vitamin D status, clinicians request 25-hydroxyvitamin D.

I live somewhere sunny, so why is my vitamin D low?

Living in a sunny place does not tell you how much vitamin D your body makes. Time spent indoors, covered skin and deeper skin pigmentation can reduce production. Diet and poor absorption can matter too. Keep protecting your skin from sunburn while you discuss the result.

What are the symptoms of low vitamin D?

Often none, which is why it is usually found on a blood test. Marked or long-standing deficiency can cause bone pain, aching or weak muscles and, in children, poor bone growth. Tiredness is common in people with low vitamin D, but a low result alone does not prove it is the cause.

Can I compare an older result in ng/mL with one in nmol/L?

Yes, if both tests measured total 25-hydroxyvitamin D. Multiply ng/mL by 2.5 to get nmol/L. For example, 20 ng/mL and 50 nmol/L are the same concentration. Check the units before treating a change in the number as a change in your vitamin D.

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Sources & further reading

Written by Dr Nirupama Sabhapathy, Specialist Clinical Pathology. Medically reviewed by Dr Joyce Jose, Specialist Clinical Pathology, on .

Catalogue information and AED price checked on 2 November 2026. Clinical suitability, preparation, collection arrangements and turnaround are confirmed for your selected test.