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Choose collection locationEndocrinology · Individual laboratory test
Free T4 measures the proportion of thyroxine circulating independently of carrier proteins. It is frequently read alongside TSH.
Clinical authorDr Nirupama SabhapathySpecialist Clinical Pathology
Understanding the test
Also called Free Thyroid Hormone (FT4)
A free T4 result complements TSH in the assessment of thyroid symptoms, including when the two appear inconsistent with the clinical picture.
Free T4 measures the fraction of thyroxine available outside its binding proteins.
The test can help a clinician interpret symptoms alongside the pituitary signal and treatment history.
Before your sample
A recent thyroid hormone dose can affect free T4. Confirm whether the draw should precede your usual dose, and tell the team about biotin. Fasting is generally unnecessary.
A clinician takes a small sample of blood from a vein. The laboratory uses serum, the liquid separated from the blood after it clots.
Smart Report
24 hoursLaboratory turnaround
The relation between free T4 and TSH can suggest where further assessment is needed. A clinician also considers recent illness and thyroid medication timing.
From sample to follow-up

Choose your location, add the test to your care bag and select a time. If you are booking for someone else, assign the test to them in checkout.
Choose collection locationLook for Thyroxine, Free (FT4) in your report. Use the sample type and collection time when comparing it with an earlier report.
Explore Smart Report
Ask how free T4 and TSH fit together and whether recent changes in medicines or illness affect the interpretation.
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Patient questions
Tell the team the name of the medicine and the time of your last dose. Follow your prescriber's instructions about the timing of the blood draw.
High-dose biotin in some hair, nail or B-complex supplements can give a falsely abnormal free T4 result with certain testing methods. Tell the team the product, dose and time you last took it so they can advise whether to pause it or reschedule.
Free T4 carries its meaning next to TSH rather than alone. When the two move in opposite directions, the problem usually lies in the thyroid itself; when both are low, a pituitary cause comes into question. TSH is the more sensitive screen, while free T4 shows how much usable hormone is actually circulating and reacts to a dose change faster.
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Written by Dr Nirupama Sabhapathy, Specialist Clinical Pathology. Medically reviewed by Dr Joyce Jose, Specialist Clinical Pathology, on .
Catalogue information and AED price checked on 2 November 2026. Clinical suitability, preparation, collection arrangements and turnaround are confirmed for your selected test.