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Choose collection locationBiochemistry · Individual laboratory test
Ionized calcium is the free calcium available to nerves, muscles and other tissues. It can answer a question that total calcium leaves open.
Clinical authorDr Nirupama SabhapathySpecialist Clinical Pathology
Understanding the test
Also called Free (Ionized) Calcium
The ionized fraction may be requested when total calcium is hard to interpret, or when a clinician needs to assess the biologically available amount more directly.
Free calcium can clarify an unexpected total when albumin is unusually low or high.
A clinician may compare ionized calcium with the hormone that regulates circulating calcium.
Before your sample
For the draw, sit quietly and breathe normally. Unusually rapid breathing can change the balance of free calcium. Follow fasting instructions for the rest of your order.
A clinician takes a small sample of blood from a vein. The laboratory uses serum, the liquid separated from the blood after it clots.
Smart Report
24 hoursLaboratory turnaround
Acid–base balance can change the proportion of calcium that is free. The result is interpreted with the reason for testing and the laboratory's interval for ionized calcium.
From sample to follow-up

Choose your location, add the test to your care bag and select a time. If you are booking for someone else, assign the test to them in checkout.
Choose collection locationLook for Calcium, Ionized in your report. Use the sample type and collection time when comparing it with an earlier report.
Explore Smart Report
Bring any paired total calcium, albumin and parathyroid measurements so the clinician can assess the same clinical episode.
Patient questions
Total calcium includes a protein-bound fraction. When albumin or blood acidity has changed, direct measurement of the free fraction can help resolve a discrepancy.
Breathing fast lowers carbon dioxide and makes blood more alkaline, which causes calcium to bind more tightly to proteins. The free fraction then reads lower, even though nothing about your calcium stores has changed.
Magnesium is needed for parathyroid hormone to be released and to act on tissues. A low free calcium that will not correct is often driven by a magnesium deficiency underneath it.
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Written by Dr Nirupama Sabhapathy, Specialist Clinical Pathology. Medically reviewed by Dr Joyce Jose, Specialist Clinical Pathology, on .
Catalogue information and AED price checked on 2 November 2026. Clinical suitability, preparation, collection arrangements and turnaround are confirmed for your selected test.