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Choose collection locationHormones & Fertility · Individual laboratory test
Total testosterone measures the combined bound and free hormone. A timed result can help investigate symptoms of androgen deficiency or excess.
Clinical authorDr Nirupama SabhapathySpecialist Clinical Pathology
Understanding the test
Also called Total Testosterone
A total testosterone measurement may help investigate symptoms of androgen deficiency or excess or follow prescribed treatment.
Total testosterone measures the combined free and protein-bound fractions.
A clinician may request another morning sample when the first result is low.
Before your sample
Confirm a morning appointment when investigating a low level. If you use testosterone treatment, ask your prescriber when the sample should be taken in relation to the dose.
A clinician takes a small sample of blood from a vein. The laboratory uses serum, the liquid separated from the blood after it clots.
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24 hoursLaboratory turnaround
Time of day, illness and hormone treatment can affect the concentration. A diagnosis or treatment change generally needs more context than one isolated result.
From sample to follow-up

Choose your location, add the test to your care bag and select a time. If you are booking for someone else, assign the test to them in checkout.
Choose collection locationLook for Testosterone, Total in your report. Use the sample type and collection time when comparing it with an earlier report.
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Discuss the time of collection and timing of any prescribed testosterone before drawing conclusions from a single value.
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Patient questions
Levels can vary with time of day, illness and other factors. A repeat measurement under comparable conditions helps establish whether the change persists.
Testosterone follows a daily rhythm, running highest in the earlier hours and falling away later. A sample taken in the afternoon can read lower simply because of the time. Taking it in the morning, and at a similar time on each occasion, makes results comparable and reduces unnecessary repeat testing.
They show where a low testosterone comes from. A raised LH points to the testes not responding despite a strong signal, while a low or normal LH with low testosterone points to the pituitary or hypothalamus not sending the instruction. The two situations are investigated and managed differently.
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Total testosterone, LH and FSH from one blood sample, to assess testosterone levels and the signals that drive them.

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Written by Dr Nirupama Sabhapathy, Specialist Clinical Pathology. Medically reviewed by Dr Joyce Jose, Specialist Clinical Pathology, on .
Catalogue information and AED price checked on 2 November 2026. Clinical suitability, preparation, collection arrangements and turnaround are confirmed for your selected test.